Mining Single Nucleotide Polymorphism (SNP) Markers for Accurate Genotype Identification and Diversity Analysis of Chinese Jujube (Ziziphus jujuba Mill.) Germplasm

Chinese jujube (Ziziphus jujuba Mill.) is an economically important fruit tree with outstanding adaptability to marginal lands and a broad range of climate conditions. There are over 800 cultivars, mostly landraces from China. However, high rate mislabeling in germplasm restricts the sharing information materials among researchers hampers use breeding. In present study, we developed large panel single nucleotide polymorphism (SNP) markers validated 288 SNPs by genotyping 114 accessions germplasm. The validation resulted designation set 192 polymorphic SNP that revealed synonymous collection Ningxia, A total 17 groups duplicates were detected, encompassing 49 cultivars. Model-based population stratification two groups, core members showed significant genetic differentiation (Fst = 0.16). results supported hypothesis cultivated had multiple origins regions domestication. Neighbor-Joining dendrogram further this comprised sub-groups, each including 1-13 closely related Parentage analysis cultivars known pedigree proved efficacy using these for parentage verification. subset 96 index was selected future downstream application gene bank management, verification pedigrees breeding programs, quality control propagation planting support traceability authentication products.